منابع مشابه
Single-molecule imaging of full protein synthesis by immobilized ribosomes
How folding of proteins is coupled to their synthesis remains poorly understood. Here, we apply single-molecule fluorescence imaging to full protein synthesis in vitro. Ribosomes were specifically immobilized onto glass surfaces and synthesis of green fluorescent protein (GFP) was achieved using modified commercial Protein Synthesis using Recombinant Elements that lacked ribosomes but contained...
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The hitherto elusive silaisocyanoacetylene molecule (HCCNSi)-a member of the silaisocyanide family-has been synthesized for the first time through the reaction of the silicon nitride radical (SiN) with acetylene (C(2)H(2)) in the gas phase under single collision conditions. Compared to the isoelectronic reaction of the cyano radical (CN) with acetylene, the replacement of the carbon atom in the...
متن کاملSingle Molecule Fluorescence Microscopy and its Applications to Single Molecule Sequencing by Cyclic Synthesis
1.0. Introduction 2.0. Background 2.1. Single Molecule Detection 2.2. Total Internal Reflection 2.3. FRET Theory 3.0. DNA Sequencing by Cyclic synthesis 3.1. Motivation 3.2. Surface Treatment 3.3. Polymerase Kinetics 3.4. Sequencing Strategies 3.4.1. Cyclic Synthesis using FRET 3.4.2. Real Time Imaging 3.4.3. Non FRET Imaging 3.4.4. Cleavable Linkers 3.4.5. Cleavable Terminators 3.4.6. Multi-Co...
متن کاملProtein-protein unbinding induced by force: single-molecule studies.
Experiments in which two specifically interacting protein molecules are dissociated by external force have yielded new insights into mechanisms involved in cell adhesion, leukocyte rolling, regulation of integrin activity, antigen-antibody interactions and other protein-mediated reactions contingent upon molecular recognition. Another important aspect of force-induced protein-protein unbinding ...
متن کاملSequence-specific peptide synthesis by an artificial small-molecule machine.
The ribosome builds proteins by joining together amino acids in an order determined by messenger RNA. Here, we report on the design, synthesis, and operation of an artificial small-molecule machine that travels along a molecular strand, picking up amino acids that block its path, to synthesize a peptide in a sequence-specific manner. The chemical structure is based on a rotaxane, a molecular ri...
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ژورنال
عنوان ژورنال: Genome Biology
سال: 2006
ISSN: 1465-6906
DOI: 10.1186/gb-2006-7-6-221